Blastocyst-like structures generated solely from stem cells

  • 1.

    Wennekamp, S., Mesecke, S., Nédélec, F. Hiiragi, T. A self-organization framework for symmetry breaking in the mammalian embryo. Nat. Rev. Mol. Cell Biol. 14, 452–459 (2013).

  • 2.

    Tanaka, S., Kunath, T., Hadjantonakis, A. K., Nagy, A. Rossant, J. Promotion of trophoblast stem cell proliferation by FGF4. Science 282, 2072–2075 (1998).

  • 3.

    Ying, Q.-L. et al. The ground state of embryonic stem cell self-renewal. Nature 453, 519–523 (2008).

  • 4.

    van den Brink, S. C. et al. Symmetry breaking, germ layer specification and axial organisation in aggregates of mouse embryonic stem cells. Development 141, 4231–4242 (2014).

  • 5.

    Harrison, S. E., Sozen, B., Christodoulou, N., Kyprianou, C. Zernicka-Goetz, M. Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro. Science 356, eaal1810 (2017).

  • 6.

    Rai, A. Cross, J. C. Three-dimensional cultures of trophoblast stem cells autonomously develop vascular-like spaces lined by trophoblast giant cells. Dev. Biol. 398, 110–119 (2015).

  • 7.

    Rivron, N. C. et al. Tissue deformation spatially modulates VEGF signaling and angiogenesis. Proc. Natl Acad. Sci. USA 109, 6886–6891 (2012).

  • 8.

    Vrij, E. et al. Directed assembly and development of material-free tissues with complex architectures. Adv. Mater. 28, 4032–4039 (2016).

  • 9.

    Manejwala, F., Kaji, E. Schultz, R. M. Development of activatable adenylate cyclase in the preimplantation mouse embryo and a role for cyclic AMP in blastocoel formation. Cell 46, 95–103 (1986).

  • 10.

    Kemp, C., Willems, E., Abdo, S., Lambiv, L. Leyns, L. Expression of all Wnt genes and their secreted antagonists during mouse blastocyst and postimplantation development. Dev. Dyn. 233, 1064–1075 (2005).

  • 11.

    Ralston, A. Rossant, J. Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo. Dev. Biol. 313, 614–629 (2008).

  • 12.

    McDole, K. Zheng, Y. Generation and live imaging of an endogenous Cdx2 reporter mouse line. Genesis 50, 775–782 (2012).

  • 13.

    Kubaczka, C. et al. Derivation and maintenance of murine trophoblast stem cells under defined conditions. Stem Cell Reports 2, 232–242 (2014).

  • 14.

    Plusa, B., Piliszek, A., Frankenberg, S., Artus, J. Hadjantonakis, A.-K. Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development 135, 3081–3091 (2008).

  • 15.

    Simmons, D. G., Fortier, A. L. Cross, J. C. Diverse subtypes and developmental origins of trophoblast giant cells in the mouse placenta. Dev. Biol. 304, 567–578 (2007).

  • 16.

    Red-Horse, K. et al. Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface. J. Clin. Invest. 114, 744–754 (2004).

  • 17.

    Latos, P. A. Hemberger, M. From the stem of the placental tree: trophoblast stem cells and their progeny. Development 143, 3650–3660 (2016).

  • 18.

    McConaha, M. E., Eckstrum, K., An, J., Steinle, J. J. Bany, B. M. Microarray assessment of the influence of the conceptus on gene expression in the mouse uterus during decidualization. Reproduction 141, 511–527 (2011).

  • 19.

    Ohnishi, Y. et al. Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages. Nat. Cell Biol. 16, 27–37 (2014).

  • 20.

    Gotoh, N. et al. The docking protein FRS2alpha is an essential component of multiple fibroblast growth factor responses during early mouse development. Mol. Cell. Biol. 25, 4105–4116 (2005).

  • 21.

    Saba-El-Leil, M. K. et al. An essential function of the mitogen-activated protein kinase Erk2 in mouse trophoblast development. EMBO Rep. 4, 964–968 (2003).

  • 22.

    Arman, E., Haffner-Krausz, R., Chen, Y., Heath, J. K. Lonai, P. Targeted disruption of fibroblast growth factor (FGF) receptor 2 suggests a role for FGF signaling in pregastrulation mammalian development. Proc. Natl Acad. Sci. USA 95, 5082–5087 (1998).

  • 23.

    Papanayotou, C. Collignon, J. Activin/Nodal signalling before implantation: setting the stage for embryo patterning. Phil. Trans. R. Soc. Lond. B 369, 1–8 (2014).

  • 24.

    Mesnard, D. Constam, D. B. Imaging proprotein convertase activities and their regulation in the implanting mouse blastocyst. J. Cell Biol. 191, 129–139 (2010).

  • 25.

    Gardner, R. L. Flow of cells from polar to mural trophectoderm is polarized in the mouse blastocyst. Hum. Reprod. 15, 694–701 (2000).

  • 26.

    Gardner, R. L., Papaioannou, V. E. Barton, S. C. Origin of the ectoplacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass. J. Embryol. Exp. Morphol. 30, 561–572 (1973).

  • 27.

    Matsumoto, N. et al. Developmental regulation of yolk sac hematopoiesis by Kruppel-like factor 6. Blood 107, 1357–1365 (2006).

  • 28.

    DiFeo, A. et al. E-cadherin is a novel transcriptional target of the KLF6 tumor suppressor. Oncogene 25, 6026–6031 (2006).

  • 29.

    Tarkowski, A. K. Wróblewska, J. Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage. J. Embryol. Exp. Morphol. 18, 155–180 (1967).

  • 30.

    Tesar, P. J. et al. New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature 448, 196–199 (2007).

  • 31.

    Rivron, N. C. In vitro generation of blastoids from trophoblast stem cells and embryonic stem cells. Protoc. Exch. (2018).

  • 32.

    van de Wetering, M. et al. The β-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells. Cell 111, 241–250 (2002).

  • 33.

    Muraro, M. J. et al. A single-cell transcriptome atlas of the human pancreas. Cell Syst. 3, 385–394.e3. (2016).

  • 34.

    Hashimshony, T. et al. CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq. Genome Biol. 17, 77 (2016).

  • 35.

    Hashimshony, T., Wagner, F., Sher, N. Yanai, I. CEL-Seq: single-cell RNA-Seq by multiplexed linear amplification. Cell Reports 2, 666–673 (2012).

  • 36.

    Grün, D. et al. Single-cell messenger RNA sequencing reveals rare intestinal cell types. Nature 525, 251–255 (2015).

  • 37.

    Anders, S. Huber, W. Differential expression analysis for sequence count data. Genome Biol. 11, R106 (2010).

  • 38.

    Dennis, G., Jr et al. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 4, 3 (2003).

  • 39.

    Eden, E., Navon, R., Steinfeld, I., Lipson, D. Yakhini, Z. GOrilla: a tool for discovery and visualization of enriched GO terms in ranked gene lists. BMC Bioinformatics 10, 48 (2009).

  • 40.

    Vrij, E. J. et al. 3D high throughput screening and profiling of embryoid bodies in thermoformed microwell plates. Lab Chip 16, 734–742 (2016).

  • 41.

    Nakamura, T. et al. SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression. Nucleic Acids Res. 43, e60 (2015).

  • 42.

    Kolodziejczyk, A. A. et al. Single cell RNA-sequencing of pluripotent states unlocks modular transcriptional variation. Cell Stem Cell 17, 471–485 (2015).

  • 43.

    Qiu, D. et al. Klf2 and Tfcp2l1, two Wnt/β-catenin targets, act synergistically to induce and maintain naive pluripotency. Stem Cell Reports 5, 314–322 (2015).

  • 44.

    Morgani, S. M. et al. Totipotent embryonic stem cells arise in ground-state culture conditions. Cell Rep. 3, 1945–1957 (2013).

  • 45.

    Hussein, S. M., Duff, E. K. Sirard, C. Smad4 and β-catenin co-activators functionally interact with lymphoid-enhancing factor to regulate graded expression of Msx2. J. Biol. Chem. 278, 48805–48814 (2003).

  • 46.

    Labbé, E. et al. Transcriptional cooperation between the transforming growth factor-β and Wnt pathways in mammary and intestinal tumorigenesis. Cancer Res. 67, 75–84 (2007).

  • Leave a Reply

    Your email address will not be published. Required fields are marked *